Issue 10, 2012
From the journal:

Analyst

Label-free fluorescent assays based on aptamer–target recognition†

Ying Tan,a   Xin Zhang,a   Yonghua Xie,a   Rui Zhao,a   Chunyan Tan*a  and  Yuyang Jiang*ab  

* Corresponding authors

a Guangdong Provincial Key Laboratory of Chemical Biology, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P. R. China
E-mail: jiangyy@sz.tsinghua.edu.cn, tancy@sz.tsinghua.edu.cn
Fax: +86-755-26032094
Tel: +86-755-26036017

b Department of Pharmacology and Pharmaceutical Sciences, School of Medicine, Tsinghua University, Beijing, P. R. China

Abstract

Label-free fluorescent assays were developed based on the competition of intramolecular DNA hybridization and aptamer–target binding. Using small molecule adenosine triphosphate (ATP) and biomacro-molecule thrombin as model targets, our design was proved to be a general method with good sensitivity and high selectivity.

Graphical abstract: Label-free fluorescent assays based on aptamer–target recognition

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Article information

DOI
https://doi.org/10.1039/C2AN16092G
Article type
Communication
Submitted
11 Nov 2011
Accepted
02 Mar 2012
First published
27 Mar 2012

Analyst, 2012,137, 2309-2312

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Label-free fluorescent assays based on aptamer–target recognition

Y. Tan, X. Zhang, Y. Xie, R. Zhao, C. Tan and Y. Jiang, Analyst, 2012, 137, 2309 DOI: 10.1039/C2AN16092G

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