Issue 6, 2012
From the journal:

Analyst

In situ monitoring of a trace intermediate during DNA phosphorylation by T4 polynucleotide kinase for transient kinetic studies†

Hiroyuki Furusawa,a   Kensuke Uemura,a   Hiroshi Yoshiminea  and  Yoshio Okahata*a  

* Corresponding authors

a Department of Biomolecular Engineering, Tokyo Institute of Technology, B-53 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan
E-mail: yokahata@bio.titech.ac.jp
Fax: +81-45-924-5836

Abstract

Formation and decomposition of the enzyme–substrate (ES) complex during phosphorylation by T4 polynucleotide kinase (T4 PNK) of dsDNAs were monitored using a highly sensitive quartz crystal microbalance (QCM) to determine kinetic parameters, which were characterised in comparison with those of other enzymes such as DNA polymerase and exo- and endo-nucleases.

Graphical abstract: In situ monitoring of a trace intermediate during DNA phosphorylation by T4 polynucleotide kinase for transient kinetic studies

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Article information

DOI
https://doi.org/10.1039/C2AN16273C
Article type
Communication
Submitted
19 Dec 2011
Accepted
19 Jan 2012
First published
20 Jan 2012

Analyst, 2012,137, 1334-1337

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In situ monitoring of a trace intermediate during DNA phosphorylation by T4 polynucleotide kinase for transient kinetic studies

H. Furusawa, K. Uemura, H. Yoshimine and Y. Okahata, Analyst, 2012, 137, 1334 DOI: 10.1039/C2AN16273C

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