Issue 16, 2012

A fluorescent probe directly detect peroxynitrite based on boronateoxidation and its applications for fluorescence imaging in living cells

Abstract

We present the design, synthesis, spectroscopy, and biological applications of PyBor, a new type of fluorescent probe for peroxynitrite detection in aqueous solution and living cells. The probe employs pyrene as the fluorophore, and is equipped with a chemically responsive unit boronate. The fluorescent probe can selectively detect peroxynitrite with fluorimetric determination and high-performance liquid chromatography analyses in aqueous solution and RAW264.7 cells intracellular free extracts. We also study our probe to time dependent peroxynitrite release from 3-morpholinylsydnonimine hydrochloride. Confocal microscopy experiments using mouse macrophage cell line RAW264.7 show that PyBor is able to detect the different intracellular peroxynitrite levels. In addition, we have performed quantum chemical calculations with TD-DFT/M06/TZVP level with COSMO solvation model basis sets using a suite of Gaussian 09 programs to provide insights into the structure optical properties of PyBor and PyOH.

Graphical abstract: A fluorescent probe directly detect peroxynitrite based on boronate oxidation and its applications for fluorescence imaging in living cells

Supplementary files

Article information

Article type
Paper
Submitted
21 Feb 2012
Accepted
25 May 2012
First published
30 May 2012

Analyst, 2012,137, 3740-3749

A fluorescent probe directly detect peroxynitrite based on boronate oxidation and its applications for fluorescence imaging in living cells

F. Yu, P. Song, P. Li, B. Wang and K. Han, Analyst, 2012, 137, 3740 DOI: 10.1039/C2AN35246J

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