Chemiluminescence determination of primidone in pharmaceuticals and human fluids

Abstract

A chemiluminescence method using a flow injection system has been developed for the determination of primidone (PRI). The method is based on the fact that the weak chemiluminescence produced in the reaction of tris(1,10-phenanthroline)ruthenium(II) and acidic Ce(IV) is enhanced in the presence of PRI. In this method, the detection limit and linear dynamic range were 0.43 μg mL⁻¹ and 0.5–20 μg mL⁻¹, respectively. In the developed method, the detection limit was lower than the therapeutic concentration of PRI. The relative standard deviation for six repeated measurements of 10.0 μg mL⁻¹ of PRI was 4.3%, and the sampling rate for analysis was 180 samples per hour. The proposed method was successfully applied to the analysis of commercial tablets containing the drug, and the results were in accordance with those obtained by the reference method. The proposed method was further applied to the determination of PRI in spiked human plasma and spiked saliva, and good recoveries were obtained for the quantification of PRI in saliva samples. In this work, the mean recovery of PRI in all matrices was 100.4 ± 4.8%.