An efficient and simple method for site-selective modification of O6-methyl-2′-deoxyguanosine in DNA

Kazumitsu Onizuka; Takamasa Nishioka; Zhichun Li; Daichi Jitsuzaki; Yosuke Taniguchi; Shigeki Sasaki

doi:10.1039/C2CC17621A

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

An efficient and simple method for site-selective modification of O⁶-methyl-2′-deoxyguanosine in DNA†

Kazumitsu Onizuka,^ab Takamasa Nishioka,^a Zhichun Li,^ab Daichi Jitsuzaki,^a Yosuke Taniguchi^ab and Shigeki Sasaki*^ab

Author affiliations

* Corresponding authors

^a Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
E-mail: saski@phar.kyushu-u.ac.jp
Fax: +81-92-642-6615
Tel: +81-92-642-6615

^b CREST, Japan Science and Technology Agency, 4-1-8 Motomachi, Kawaguchi, Saitama 332-0012, Japan

Abstract

O ⁶-Methyl-2′-deoxyguanosine (O⁶-Me-dG) is a mutagenic nucleotide in DNA. O⁶-Me-dG in DNA was rapidly and selectively modified by a functionality transfer reaction using the ODN incorporating 6-S-functionalized thioguanosine. Subsequent labelling of O⁶-Me-dG with the fluorescent FAM or biotin group via click chemistry has permitted the sensitive and selective detection of O⁶-Me-dG in DNA.

Download options Please wait...

Supplementary files

Supplementary information PDF (679K)

Article information

DOI: https://doi.org/10.1039/C2CC17621A
Article type: Communication
Submitted: 06 Dec 2011
Accepted: 01 Mar 2012
First published: 01 Mar 2012

Download Citation

Chem. Commun., 2012,48, 3969-3971

Permissions

Request permissions

An efficient and simple method for site-selective modification of O⁶-methyl-2′-deoxyguanosine in DNA

K. Onizuka, T. Nishioka, Z. Li, D. Jitsuzaki, Y. Taniguchi and S. Sasaki, Chem. Commun., 2012, 48, 3969 DOI: 10.1039/C2CC17621A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Social activity

Fetching data from CrossRef.
This may take some time to load.

Chemical Communications