Issue 42, 2012
From the journal:

Chemical Communications

Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging†

Antonella Battisti,*a   Michelle A. Digman,b   Enrico Gratton,b   Barbara Storti,a   Fabio Beltrama  and  Ranieri Bizzarri‡*a  

* Corresponding authors

a NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, Piazza San Silvestro 12, 56127 Pisa, Italy
E-mail: antonella.battisti@sns.it, r.bizzarri@sns.it
Fax: +39 050509417
Tel: +39 050509767

b Laboratory for Fluorescence Dynamics, University of California, Irvine, USA

Abstract

A versatile pH-dependent fluorescent protein was applied to intracellular pH measurements by means of the phasor approach to fluorescence lifetime imaging. By this fit-less method we obtain intracellular pH maps under resting or altered physiological conditions by single-photon confocal or two-photon microscopy.

Graphical abstract: Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging

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Article information

DOI
https://doi.org/10.1039/C2CC30373F
Article type
Communication
Submitted
16 Jan 2012
Accepted
03 Apr 2012
First published
04 Apr 2012

Chem. Commun., 2012,48, 5127-5129

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Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging

A. Battisti, M. A. Digman, E. Gratton, B. Storti, F. Beltram and R. Bizzarri, Chem. Commun., 2012, 48, 5127 DOI: 10.1039/C2CC30373F

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