Issue 39, 2012

Fluorescence imaging of potassium ions in living cells using a fluorescent probe based on a thrombin binding aptamer–peptide conjugate

Abstract

When a biotinylated FRET probe based on a peptide–thrombin binding aptamer conjugate was introduced together with streptavidin and biotinylated nuclear export signal peptide into HeLa cells, the resulting ternary complex enabled visualization of K+ concentration changes in the cell.

Graphical abstract: Fluorescence imaging of potassium ions in living cells using a fluorescent probe based on a thrombin binding aptamer–peptide conjugate

Supplementary files

Article information

Article type
Communication
Submitted
24 Jan 2012
Accepted
20 Mar 2012
First published
20 Mar 2012

Chem. Commun., 2012,48, 4740-4742

Fluorescence imaging of potassium ions in living cells using a fluorescent probe based on a thrombin binding aptamer–peptide conjugate

K. Ohtsuka, S. Sato, Y. Sato, K. Sota, S. Ohzawa, T. Matsuda, K. Takemoto, N. Takamune, B. Juskowiak, T. Nagai and S. Takenaka, Chem. Commun., 2012, 48, 4740 DOI: 10.1039/C2CC30536D

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