Issue 50, 2012
From the journal:

Chemical Communications

Programmable protein–protein conjugation via DNA-based self-assembly†

Josui Shimada,a   Tatsuo Maruyama,*b   Momoko Kitaoka,a   Hisao Yoshinaga,a   Koji Nakano,a   Noriho Kamiyaac  and  Masahiro Goto*ac  

* Corresponding authors

a Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Fukuoka 819-0395, Japan
E-mail: m-goto@mail.cstm.kyushu-u.ac.jp
Fax: +81-(0)92-802-2810
Tel: +81-(0)92-802-2810

b Department of Chemical Science and Engineering, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan
E-mail: tmarutcm@crystal.kobe-u.ac.jp
Fax: +81-(0)78-803-6070

c Center for Future Chemistry, Center for Future Chemistry, Kyushu University, 744 Moto-oka, Fukuoka 819-0395, Japan
Fax: +81-(0)92-802-2810
Tel: +81-(0)92-802-2810

Abstract

Protein molecules were precisely arrayed on a designable DNA scaffold close to each other using a DNA aptamer. By adding a chemical cross-linker, the neighboring protein molecules were effectively and covalently cross-linked to each other without losing their activities.

Graphical abstract: Programmable protein–protein conjugation via DNA-based self-assembly

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Article information

DOI
https://doi.org/10.1039/C2CC30618B
Article type
Communication
Submitted
28 Jan 2012
Accepted
21 Feb 2012
First published
22 Feb 2012

Chem. Commun., 2012,48, 6226-6228

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Programmable protein–protein conjugation via DNA-based self-assembly

J. Shimada, T. Maruyama, M. Kitaoka, H. Yoshinaga, K. Nakano, N. Kamiya and M. Goto, Chem. Commun., 2012, 48, 6226 DOI: 10.1039/C2CC30618B

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