Issue 43, 2012
From the journal:

Chemical Communications

Ionic liquids promote PCR amplification of DNA†

Yugang Shi,ab   Yen-Liang Liu,a   Peng-Yeh Lai,c   Ming-Chung Tseng,a   Min-Jen Tseng,c   Yudong Lib  and  Yen-Ho Chu*a  

* Corresponding authors

a Department of Chemistry and Biochemistry, National Chung Cheng University, Minhsiung, Chiayi 62102, Taiwan, ROC
E-mail: cheyhc@ccu.edu.tw
Fax: +886 52721040
Tel: +886 52428148

b College of Food Science and Biotechnology, Zhejiang Provincial Key Laboratory of Food Safety, Zhejiang Gongshang University, Hangzhou, PR China

c Institute of Molecular Biology, National Chung Cheng University, Minhsiung, Chiayi 62102, Taiwan, ROC

Abstract

A bicyclic imidazolium ionic liquid (4d), [b-4C-im][Br], was found to be highly effective not only for promoting PCR of GC-rich DNA by minimizing non-specific amplification, but also for facilitating PCR of normal-GC DNA under mild conditions.

Graphical abstract: Ionic liquids promote PCR amplification of DNA

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Article information

DOI
https://doi.org/10.1039/C2CC31740K
Article type
Communication
Submitted
08 Mar 2012
Accepted
05 Apr 2012
First published
10 Apr 2012

Chem. Commun., 2012,48, 5325-5327

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Ionic liquids promote PCR amplification of DNA

Y. Shi, Y. Liu, P. Lai, M. Tseng, M. Tseng, Y. Li and Y. Chu, Chem. Commun., 2012, 48, 5325 DOI: 10.1039/C2CC31740K

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