It is demonstrated that engineered arginase with a single protruded site of cysteine deliberately placed away from its active centre by site-directed mutagenesis can facilitate its attachment on a gold-nanoparticle surface with atomic precision, resulting in no apparent loss in enzymatic activity.
G. K. Lee, S. Kwok, C. H. Yu, K. Tam, H. Chong, Y. Leung and S. C. E. Tsang, Chem. Commun., 2012, 48, 7693 DOI: 10.1039/C2CC32863A
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