Issue 100, 2012
From the journal:

Chemical Communications

Simple and sensitive fluorescence detection of the RNA endonuclease activity of mammalian argonaute2 protein based on an RNA molecular beacon†

Feng Li,ab   Peng Li,b   Limin Yanga  and  Bo Tang*a  

* Corresponding authors

a College of Chemistry, Chemical Engineering and Materials Science, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Ministry of Education, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan 250014, China
E-mail: tangb@sdnu.edu.cn
Fax: +86-531-86180017

b College of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao 266042, China

Abstract

A new strategy for determining the RNA endonuclease activity of mammalian argonaute2 (Ago2) protein has been developed, which combines the unique cleavage function of Ago2 protein with an RNA molecular beacon (RMB). Through the fluorescence restoration of the RMB, simple and sensitive detection of Ago2 is achieved.

Graphical abstract: Simple and sensitive fluorescence detection of the RNA endonuclease activity of mammalian argonaute2 protein based on an RNA molecular beacon

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Article information

DOI
https://doi.org/10.1039/C2CC36404B
Article type
Communication
Submitted
03 Sep 2012
Accepted
01 Nov 2012
First published
02 Nov 2012

Chem. Commun., 2012,48, 12192-12194

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Simple and sensitive fluorescence detection of the RNA endonuclease activity of mammalian argonaute2 protein based on an RNA molecular beacon

F. Li, P. Li, L. Yang and B. Tang, Chem. Commun., 2012, 48, 12192 DOI: 10.1039/C2CC36404B

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