Issue 8, 2012
From the journal:

RSC Advances

Biotinylated boronic acid fluorophore conjugates: Quencher elimination strategy for imaging and saccharide detection

François D'Hooge,†ab   Souad A. Elfeky,†abc   Stephen E. Flower,b   Sofia I. Pascu,b   A. Toby A. Jenkins,b   Jean M. H. van den Elsen,d   Tony D. James*b  and  John S. Fossey*a  

* Corresponding authors

a School of Chemistry, University of Birmingham, Edgbaston, Birmingham, West Midlands, UK
E-mail: j.s.fossey@bham.ac.uk

b University of Bath, Department of Chemistry, Claverton Down, Bath, UK
E-mail: t.d.james@bath.ac.uk

c Cairo University-National Institute of Laser Enhanced Science, Egypt

d University of Bath, Department of Biology and Biochemistry, Bath, UK

Abstract

A biotinylated boronic acid fluorophore conjugate bound to a diol-quencher via a boronic ester linkage demonstrated a fluorescence increase upon exposure to model saccharides. The sensing motif was attached to SuperAvidin microspheres where the sensing regime was imaged by fluorescence microscopy.

Graphical abstract: Biotinylated boronic acid fluorophore conjugates: Quencher elimination strategy for imaging and saccharide detection

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Article information

DOI
https://doi.org/10.1039/C2RA00542E
Article type
Paper
Submitted
01 Aug 2011
Accepted
22 Nov 2011
First published
24 Feb 2012

RSC Adv., 2012,2, 3274-3280

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Biotinylated boronic acid fluorophore conjugates: Quencher elimination strategy for imaging and saccharide detection

F. D'Hooge, S. A. Elfeky, S. E. Flower, S. I. Pascu, A. T. A. Jenkins, J. M. H. V. D. Elsen, T. D. James and J. S. Fossey, RSC Adv., 2012, 2, 3274 DOI: 10.1039/C2RA00542E

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