Issue 9, 2012
From the journal:

Chemical Science

Genetically encoded alkenyl–pyrrolysine analogues for thiol–ene reaction mediated site-specific protein labeling†

Yiming Li,abc   Maiyun Yang,a   Yichao Huang,b   Xiaoda Song,a   Lei Liu*b  and  Peng R. Chen*a  

* Corresponding authors

a Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Peking-Tsinghua Center for Life Sciences, Department of Chemical Biology, Peking University, Beijing, 100871, P. R. China
E-mail: pengchen@pku.edu.cn
Fax: +86 10-62767433
Tel: +86 10-62755773

b Department of Chemistry, Tsinghua University, Beijing, 100084, P. R. China
E-mail: lliu@mail.tsinghua.edu.cn
Tel: +86 10-62780027

c School of Medical Engineering, Hefei University of Technology, Hefei, 230009, P. R. China

Abstract

A series of alkene-bearing pyrrolysine analogues were synthesized and subsequently incorporated into proteins at two sites by a mutant PylRS–tRNA pair with excellent efficiency. This strategy allowed the site-specific labeling of proteins carrying single or double genetically encoded alkene handles via bioorthogonal thiol–ene ligation reactions.

Graphical abstract: Genetically encoded alkenyl–pyrrolysine analogues for thiol–ene reaction mediated site-specific protein labeling

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Article information

DOI
https://doi.org/10.1039/C2SC20433A
Article type
Edge Article
Submitted
09 Apr 2012
Accepted
19 Jun 2012
First published
19 Jun 2012

Chem. Sci., 2012,3, 2766-2770

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Genetically encoded alkenyl–pyrrolysine analogues for thiol–ene reaction mediated site-specific protein labeling

Y. Li, M. Yang, Y. Huang, X. Song, L. Liu and P. R. Chen, Chem. Sci., 2012, 3, 2766 DOI: 10.1039/C2SC20433A

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