Issue 11, 2013

A highly selective turn-on fluorescent sensor for Cu(ii) based on an NSe2 chelating moiety and its application in living cell imaging

Abstract

In this study, a boron-dipyrromethene (BODIPY)-based fluorescent chemosensor CBS was developed for metal ion sensing. It was found that CBS containing an NSe2 moiety exhibited high selectivity for Cu2+ detection while CBS in the presence of Cu2+ displayed significant fluorescence enhancement. However, the metal ions Ag+, Ca2+, Co2+, Cr3+, Fe2+, Fe3+, Hg2+, K+, Mg2+, Mn2+, Ni2+, Pb2+, and Zn2+ produced only minor changes in the fluorescence values of the system. The binding constant (Ka) of Cu2+ binding to CBS was found to be 7.28 × 103 M−1. The binding ratio of CBS–Cu2+ complexes was determined from the Job plot to be 1 : 1. The maximum fluorescence enhancement caused by Cu2+ binding to CBS was observed over the pH range 5.0–9.0. Additionally, the methyl thiazolyl tetrazolium (MTT) assay demonstrated the CBS to have low cytotoxicity. Confocal fluorescence microscopy imaging using RAW264.7 cells showed that CBS could be used as an effective fluorescent probe for detecting Cu2+ in living cells.

Graphical abstract: A highly selective turn-on fluorescent sensor for Cu(ii) based on an NSe2 chelating moiety and its application in living cell imaging

Supplementary files

Article information

Article type
Paper
Submitted
06 Feb 2013
Accepted
28 Mar 2013
First published
28 Mar 2013

Analyst, 2013,138, 3264-3270

A highly selective turn-on fluorescent sensor for Cu(II) based on an NSe2 chelating moiety and its application in living cell imaging

C. Chou, S. Liu and S. Wu, Analyst, 2013, 138, 3264 DOI: 10.1039/C3AN00286A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements