Electrochemical detection of dopamine based on pre-concentration by graphenenanosheets

Abstract

Herein, graphene nanosheets (GNS) were synthesized, by a green and facile method based on reduction by glucose, and characterized. Afterwards, a carbon paste electrode (CPE) was modified with GNS by casting and drying GNS on top of the CPE (CPE/GNS). The behavior of the CPE/GNS towards dopamine (DA) and ascorbic acid (AA) was investigated by electrochemical methods and the obtained results showed that the CPE/GNS had adsorbed only DA. Based on this behavior, the DA molecules were pre-concentrated on top of the CPE/GNS, followed by stripping in DA free solution. Subsequent to experimental and instrumental optimization, a calibration curve from 2.0 × 10⁻⁶ to 1.0 × 10⁻³ M DA, r² = 0.99 (±0.01), with detection limit (DL) = 8.5 × 10⁻⁷ M DA, sensitivity = 15.4 (±0.94) μA, and RSD = 6.1 was observed in the presence of 1.0 × 10⁻³ M AA. Finally, the performance of the CPE/GNS was successfully tested in a pharmaceutical sample. This work provides a promising strategy for DA detection in the presence of biological interferences, e.g. AA, with high sensitivity and simple characteristics.