Issue 44, 2013
From the journal:

Chemical Communications

In-cell covalent labeling of reactive His-tag fused proteins†

Shohei Uchinomiya,a   Hiroshi Nonaka,b   Sho Wakayama,a   Akio Ojidac  and  Itaru Hamachi*ad  

* Corresponding authors

a Department of Synthetic Chemistry and Biological Chemistry, Kyoto University, Katsura, Kyoto 615-8510, Japan
E-mail: ihamachi@sbchem.kyoto-u.ac.jp
Fax: +81-75-383-2759
Tel: +81-75-383-2754

b INAMORI Frontier Research Center, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, Japan

c Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, Japan

d Japan Science and Technology Agency (JST), CREST, 5 Sanbancho, Chiyoda-ku, Tokyo, Japan

Abstract

A new method for in-cell protein labeling was developed. This method employed a binding-induced nucleophilic reaction between the Cys-appended His-tag and the Ni(II)–NTA containing an α-chloroacetamide. Using this method, not only labeling of His-tag fused proteins but also the detection of a protein–protein interaction was achieved inside living cells.

Graphical abstract: In-cell covalent labeling of reactive His-tag fused proteins

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Article information

DOI
https://doi.org/10.1039/C3CC41979G
Article type
Communication
Submitted
18 Mar 2013
Accepted
11 Apr 2013
First published
11 Apr 2013

Chem. Commun., 2013,49, 5022-5024

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In-cell covalent labeling of reactive His-tag fused proteins

S. Uchinomiya, H. Nonaka, S. Wakayama, A. Ojida and I. Hamachi, Chem. Commun., 2013, 49, 5022 DOI: 10.1039/C3CC41979G

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