We have developed a fluorogenic NAD(P)+ detection method using 2-acetylbenzofuran. The reaction of NAD(P)+ with 2-acetylbenzofuran produced a fluorescent product, allowing the highly-sensitive and quick detection of NAD(P)+. This method was successfully applied to the detection of P450 substrates in the microtiter-plate format.
T. Moriya, A. Kawamata, Y. Takahashi, Y. Iwabuchi and N. Kanoh, Chem. Commun., 2013, 49, 11500 DOI: 10.1039/C3CC47264G
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