Issue 22, 2013

Dissecting the structural determinants for the difference in mechanical stability of silk and amyloid beta-sheet stacks

Abstract

Stacking of β-sheets results in a protein super secondary structure with remarkable mechanical properties. β-Stacks are the determinants of a silk fiber's resilience and are also the building blocks of amyloid fibrils. While both silk and amyloid-type crystals are known to feature a high resistance against rupture, their structural and mechanical similarities and particularities are yet to be fully understood. Here, we systematically compare the rupture force and stiffness of amyloid and spider silk poly-alanine β-stacks of comparable sizes using Molecular Dynamics simulations. We identify the direction of force application as the primary determinant of the rupture strength; β-sheets in silk are orientated along the fiber axis, i.e. the pulling direction, and consequently require high forces in the several nanoNewton range for shearing β-strands apart, while β-sheets in amyloid are oriented vertically to the fiber, allowing a zipper-like rupture at sub-nanoNewton forces. A secondary factor rendering amyloid β-stacks softer and weaker than their spider silk counterparts is the sub-optimal side-chain packing between β-sheets due to the sequence variations of amyloid-forming proteins as opposed to the perfectly packed poly-alanine β-sheets of silk. Taken together, amyloid fibers can reach the stiffness of silk fibers in spite of their softer and weaker β-sheet arrangement as they are missing a softening amorphous matrix.

Graphical abstract: Dissecting the structural determinants for the difference in mechanical stability of silk and amyloid beta-sheet stacks

Supplementary files

Article information

Article type
Paper
Submitted
08 Jan 2013
Accepted
10 Apr 2013
First published
11 Apr 2013

Phys. Chem. Chem. Phys., 2013,15, 8765-8771

Dissecting the structural determinants for the difference in mechanical stability of silk and amyloid beta-sheet stacks

S. Xiao, S. Xiao and F. Gräter, Phys. Chem. Chem. Phys., 2013, 15, 8765 DOI: 10.1039/C3CP00067B

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