Issue 10, 2013

The DNA binding site specificity and antiproliferative property of ternary Pt(ii) and Zn(ii) complexes of phenanthroline and N,N′-ethylenediaminediacetic acid

Abstract

The binding site specificity of the ternary complexes, [M(II)(phen)(edda)] (M(II) = Pt2+ and Zn2+; phen = 1,10-phenanthroline; edda = N,N′-ethylenediaminediacetic acid), for the self-complementary oligonucleotides (ODNs), ds(C1G2C3G4A5A6T7T8C9G10C11G12)2 (ODN1) and ds(C1G2C3G4T5A6T7A8C9G10C11G12)2 (ODN2), was studied by NMR measurements. The results indicated that [Pt(II)(phen)(edda)] was partially intercalated between C3/G10 and G4/C9 base pairs of ODN1 and ODN2 in the major grooves, whereas [Zn(II)(phen)(edda)] was bound specifically to the TATA region of ODN2 in the minor groove and to the terminal G2/C11 base pair of ODN1 in the major groove. The preference for the TATA sequence over the AATT sequence in the binding of [Zn(phen)(edda)] was attributed to the wider minor groove width of the TATA sequence. The bindings of the complexes to ct-DNA were also studied by UV, CD, and fluorescence spectroscopy. Additionally, the antiproliferative property of [Pt(II)(phen)(edda)] towards MCF7 breast cancer cells and normal MCF10-A cells was compared with that of [Zn(II)(phen)(edda)].

Graphical abstract: The DNA binding site specificity and antiproliferative property of ternary Pt(ii) and Zn(ii) complexes of phenanthroline and N,N′-ethylenediaminediacetic acid

Supplementary files

Article information

Article type
Paper
Submitted
13 Nov 2012
Accepted
12 Dec 2012
First published
12 Dec 2012

Dalton Trans., 2013,42, 3337-3345

The DNA binding site specificity and antiproliferative property of ternary Pt(II) and Zn(II) complexes of phenanthroline and N,N′-ethylenediaminediacetic acid

Y. Nakamura, Y. Taruno, M. Sugimoto, Y. Kitamura, H. L. Seng, S. M. Kong, C. H. Ng and M. Chikira, Dalton Trans., 2013, 42, 3337 DOI: 10.1039/C2DT32709K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements