Synthesis and enzymatic incorporation of norbornene-modified nucleoside triphosphates for Diels–Alder bioconjugation

Juliane Schoch; Andres Jäschke

doi:10.1039/C3RA40373D

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Synthesis and enzymatic incorporation of norbornene-modified nucleoside triphosphates for Diels–Alder bioconjugation†

Juliane Schoch^a and Andres Jäschke*^a

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* Corresponding authors

^a Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg 69120, Germany
E-mail: jaeschke@uni-hd.de
Fax: +49 (0)6221-54 6430
Tel: +49 (0)6221-54 4851

Abstract

Norbornene-modified nucleoside triphosphates were synthesised and enzymatically incorporated into DNA via primer extension and polymerase chain reaction. DNA strands containing several hundred norbornene moieties were prepared and successfully labelled utilising the inverse-electron-demand Diels–Alder reaction.

This article is part of the themed collection: Nucleic acids: new life, new materials

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Article information

DOI: https://doi.org/10.1039/C3RA40373D
Article type: Communication
Submitted: 28 Sep 2012
Accepted: 24 Jan 2013
First published: 28 Jan 2013

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RSC Adv., 2013,3, 4181-4183

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Synthesis and enzymatic incorporation of norbornene-modified nucleoside triphosphates for Diels–Alder bioconjugation

J. Schoch and A. Jäschke, RSC Adv., 2013, 3, 4181 DOI: 10.1039/C3RA40373D

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Synthesis and enzymatic incorporation of norbornene-modified nucleoside triphosphates for Diels–Alder bioconjugation†

Abstract

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Synthesis and enzymatic incorporation of norbornene-modified nucleoside triphosphates for Diels–Alder bioconjugation

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