Issue 15, 2013
From the journal:

Journal of Materials Chemistry B

A gold nanoparticle-enhanced fluorescence polarization biosensor for amplified detection of T4 polynucleotide kinase activity and inhibition†

Yong Huang,*a   Jia Chen,a   Ming Shi,a   Shulin Zhao,*a   Zheng-Feng Chena  and  Hong Liang*a  

* Corresponding authors

a Ministry of Education Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, Guangxi 541004, China
E-mail: zhaoshulin001@163.com, hliang@gxnu.edu.cn, huangyong_2009@163.com
Fax: +86 773-583-2294
Tel: +86 773 5856104

Abstract

A novel fluorescence polarization (FP) nanosensor based on λ exonuclease cleavage reaction and FP enhancement effect of gold nanoparticles (AuNPs) has been developed for the detection of T4 polynucleotide kinase activity and inhibition. This FP sensor exhibits higher detection sensitivity over traditional fluorescence sensors by two orders of magnitude.

Graphical abstract: A gold nanoparticle-enhanced fluorescence polarization biosensor for amplified detection of T4 polynucleotide kinase activity and inhibition

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Article information

DOI
https://doi.org/10.1039/C3TB00025G
Article type
Communication
Submitted
07 Jan 2013
Accepted
04 Mar 2013
First published
05 Mar 2013

J. Mater. Chem. B, 2013,1, 2018-2021

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A gold nanoparticle-enhanced fluorescence polarization biosensor for amplified detection of T4 polynucleotide kinase activity and inhibition

Y. Huang, J. Chen, M. Shi, S. Zhao, Z. Chen and H. Liang, J. Mater. Chem. B, 2013, 1, 2018 DOI: 10.1039/C3TB00025G

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