Issue 21, 2014

Facile preparation of polyvinyl alcohol coated SiO2 stationary phases for high performance liquid chromatography

Abstract

A facile method to prepare a polar stationary phase for hydrophilic interaction chromatography (HILIC) was proposed by coating polyvinyl alcohol onto silica particles (PVA-Sil). A water or organic solvent-insoluble permanent PVA coating on the silica particle surface can be formed simply by dipping silica particles into a hot PVA solution and then settled from this solution, leaving a thin layer of PVA coating and frozen in a freezer. The PVA-Sil shields the silica core from solution erosion to some degree and the pH tolerance range was extended to 9.5 from 8.0 for bare silica. PVA-Sil demonstrated a good hydrophilic property for several kinds of polar compounds and ∼57000 m−1 of plate count was achieved. This method can also be extended as a universal method to prepare various stationary phases with exchangeable functionalities by doping the desired ingredient in a PVA solution.

Graphical abstract: Facile preparation of polyvinyl alcohol coated SiO2 stationary phases for high performance liquid chromatography

Supplementary files

Article information

Article type
Paper
Submitted
05 Jun 2014
Accepted
10 Aug 2014
First published
11 Aug 2014

Analyst, 2014,139, 5594-5599

Author version available

Facile preparation of polyvinyl alcohol coated SiO2 stationary phases for high performance liquid chromatography

S. Ji, F. Zhang, S. Wu, B. Yang and X. Liang, Analyst, 2014, 139, 5594 DOI: 10.1039/C4AN01021C

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