An improved strategy for RNA labelling using an alkyne-carrying dinucleotide is reported. This involves near-quantitative priming by phage RNA-polymerases followed by conjugation of different labels using click chemistry. Moreover, these transcripts bear a ligation compatible 5′-end, and thus through ligation the terminal label can be transformed to an internal one.
A. Samanta, A. Krause and A. Jäschke, Chem. Commun., 2014, 50, 1313 DOI: 10.1039/C3CC46132G
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