Issue 6, 2014
From the journal:

Chemical Communications

Preparation and affinity-based purification of caged linear DNA for light-controlled gene expression in mammalian cells†

Aoi Teraoka,a   Kanako Murakoshi,a   Kyoko Fukamauchi,a   Akinobu Z. Suzuki,a   Soichiro Watanabea  and  Toshiaki Furuta*a  

* Corresponding authors

a Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan
E-mail: furuta@biomol.sci.toho-u.ac.jp
Fax: +81-47-472-1169
Tel: +81-47-472-1169

Abstract

A facile and useful method for preparing caged DNAs was developed. The method includes a caging reaction of a linear dsDNA having a minimal sequence of protein expression with Bio-Bhc-diazo and affinity separation of the caged DNA. Effective suppression and photo-mediated restoration of protein expression were demonstrated.

Graphical abstract: Preparation and affinity-based purification of caged linear DNA for light-controlled gene expression in mammalian cells

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Article information

DOI
https://doi.org/10.1039/C3CC46607H
Article type
Communication
Submitted
29 Aug 2013
Accepted
06 Nov 2013
First published
07 Nov 2013

Chem. Commun., 2014,50, 664-666

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Preparation and affinity-based purification of caged linear DNA for light-controlled gene expression in mammalian cells

A. Teraoka, K. Murakoshi, K. Fukamauchi, A. Z. Suzuki, S. Watanabe and T. Furuta, Chem. Commun., 2014, 50, 664 DOI: 10.1039/C3CC46607H

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