Highly emissive deoxyguanosine analogue capable of direct visualization of B–Z transition

Abstract

A 2-aminothieno[3,4-d]pyrimidine G-mimic deoxyribonucleoside, ^thdG, was synthesized and incorporated readily into oligonucleotides as a versatile fluorescent guanine analogue. We demonstrate that ^thdG enables the visual detection of Z-DNA successfully based on different π-stacking of B- and Z-DNA.