Issue 27, 2014
From the journal:

Chemical Communications

Molecular inversion probe-based SPR biosensing for specific, label-free and real-time detection of regional DNA methylation†

Laura G. Carrascosa,*a   Abu Ali Ibn Sina,a   Ramkumar Palanisamy,a   Borja Sepulveda,b   Marinus A. Otte,b   Sakandar Rauf,a   Muhamad J. A. Shiddikya  and  Matt Trau*ac  

* Corresponding authors

a Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, QLD 4072, Australia

b Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC & CIBER-BBN. ICN2 Building, Campus UAB 08193 Bellaterra, Spain
E-mail: lgcarrascosa@uq.edu.au, m.trau@uq.edu.au

c School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, QLD 4072, Australia

Abstract

DNA methylation has the potential to be a clinically important biomarker in cancer. This communication reports a real-time and label-free biosensing strategy for DNA methylation detection in the cancer cell line. This has been achieved by using surface plasmon resonance biosensing combined with the highly specific molecular inversion probe based amplification method, which requires only 50 ng of bisulfite treated genomic DNA.

Graphical abstract: Molecular inversion probe-based SPR biosensing for specific, label-free and real-time detection of regional DNA methylation

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Article information

DOI
https://doi.org/10.1039/C3CC49607D
Article type
Communication
Submitted
19 Dec 2013
Accepted
11 Feb 2014
First published
11 Feb 2014

Chem. Commun., 2014,50, 3585-3588

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Molecular inversion probe-based SPR biosensing for specific, label-free and real-time detection of regional DNA methylation

L. G. Carrascosa, A. A. I. Sina, R. Palanisamy, B. Sepulveda, M. A. Otte, S. Rauf, M. J. A. Shiddiky and M. Trau, Chem. Commun., 2014, 50, 3585 DOI: 10.1039/C3CC49607D

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