Issue 37, 2014
From the journal:

Chemical Communications

Detection and quantification of the Bcr/Abl chimeric protein on biochips using LDI-TOF MS†

Seol-Hye Hong,a   Jae Il Kim,a   Hyunook Kang,a   Soojin Yoon,b   Dong-Eun Kim,b   Woong Jungc  and  Woon-Seok Yeo*a  

* Corresponding authors

a Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea
E-mail: wsyeo@konkuk.ac.kr

b Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Korea

c Department of Emergency Medicine, Kyung Hee University Hospital at Gangdong, Seoul 134-727, Korea

Abstract

The Bcr/Abl chimeric protein was captured by two antibodies, anti-Bcr on gold nanoparticles (AuNPs) and anti-Abl on a biochip, in a sandwich assay format. The presence of the Bcr/Abl in cells was then verified by amplified LDI-TOF MS signals, and relative amounts were quantified using AuNPs coated with deuterated alkanethiols as an internal standard.

Graphical abstract: Detection and quantification of the Bcr/Abl chimeric protein on biochips using LDI-TOF MS

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Article information

DOI
https://doi.org/10.1039/C4CC01332H
Article type
Communication
Submitted
20 Feb 2014
Accepted
17 Mar 2014
First published
18 Mar 2014

Chem. Commun., 2014,50, 4831-4834

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Detection and quantification of the Bcr/Abl chimeric protein on biochips using LDI-TOF MS

S. Hong, J. I. Kim, H. Kang, S. Yoon, D. Kim, W. Jung and W. Yeo, Chem. Commun., 2014, 50, 4831 DOI: 10.1039/C4CC01332H

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