Issue 50, 2014
From the journal:

Chemical Communications

Qualitative and quantitative detection of methylation at CpG sites using the fluorescein-dGTP incorporated asymmetric PCR assay strategy†

Tingting Hong,‡a   Tianlu Wang,‡a   Zhiyong He,a   Jingwei Ma,a   Heng Xiao,a   Jinguo Huang,a   Wuxiang Maoa  and  Xiang Zhou*a  

* Corresponding authors

a College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, P. R. China
E-mail: xzhou@whu.edu.cn
Fax: +86-27-68756663

Abstract

The methylation status of each CpG site can be monitored by Fl-dGTP incorporated asymmetric PCR assay. The ability of quantitative detection makes it a good choice for detecting partial methylation at CpG sites compared with others. And the monitoring is not limited to sites within PCR primers or restriction enzyme-recognition sites.

Graphical abstract: Qualitative and quantitative detection of methylation at CpG sites using the fluorescein-dGTP incorporated asymmetric PCR assay strategy

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Article information

DOI
https://doi.org/10.1039/C4CC01933D
Article type
Communication
Submitted
15 Mar 2014
Accepted
29 Apr 2014
First published
02 May 2014

Chem. Commun., 2014,50, 6653-6655

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Qualitative and quantitative detection of methylation at CpG sites using the fluorescein-dGTP incorporated asymmetric PCR assay strategy

T. Hong, T. Wang, Z. He, J. Ma, H. Xiao, J. Huang, W. Mao and X. Zhou, Chem. Commun., 2014, 50, 6653 DOI: 10.1039/C4CC01933D

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