Issue 55, 2014
From the journal:

Chemical Communications

Monitoring enzymatic ATP hydrolysis by EPR spectroscopy†

Stephan M. Hacker,‡*a   Christian Hintze,‡a   Andreas Marx*a  and  Malte Drescher*a  

* Corresponding authors

a Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstraße 10, 78457 Konstanz, Germany
E-mail: malte.drescher@uni-konstanz.de, andreas.marx@uni-konstanz.de, stephan.hacker@uni-konstanz.de

Abstract

An adenosine triphosphate (ATP) analogue modified with two nitroxide radicals is developed and employed to study its enzymatic hydrolysis by electron paramagnetic resonance spectroscopy. For this application, we demonstrate that EPR holds the potential to complement fluorogenic substrate analogues in monitoring enzymatic activity.

Graphical abstract: Monitoring enzymatic ATP hydrolysis by EPR spectroscopy

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Article information

DOI
https://doi.org/10.1039/C4CC02422B
Article type
Communication
Submitted
02 Apr 2014
Accepted
07 May 2014
First published
15 May 2014
This article is Open Access
Creative Commons BY license

Chem. Commun., 2014,50, 7262-7264

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Monitoring enzymatic ATP hydrolysis by EPR spectroscopy

S. M. Hacker, C. Hintze, A. Marx and M. Drescher, Chem. Commun., 2014, 50, 7262 DOI: 10.1039/C4CC02422B

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