Issue 56, 2014
From the journal:

Chemical Communications

A fluorescent probe for imaging symmetric and asymmetric cell division in neurosphere formation†

Seong-Wook Yun,a   Cheryl Leong,ab   Xuezhi Bi,a   Hyung-Ho Ha,c   Yuan Hong Yu,d   Yee Ling Tan,a   Gunaseelan Narayanan,d   Shvetha Sankaran,d   Jun-Young Kim,c   Srivats Hariharan,d   Sohail Ahmed*d  and  Young-Tae Chang*ac  

* Corresponding authors

a Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Singapore

b NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore

c Department of Chemistry & NUS MedChem Program of Life Sciences Institute, National University of Singapore, Singapore
E-mail: chmcyt@nus.edu.sg

d Institute of Medical Biology, Agency for Science, Technology and Research, Singapore
E-mail: sohail.ahmed@imb.a-star.edu.sg

Abstract

We report here a novel fluorescent chemical probe CDy5 which stains distinct neural stem/progenitor cells (NSPCs) by binding to acid ceramidase in mouse neurospheres. CDy5 is distributed evenly or unevenly to the daughter cells during multiple mitoses enabling the live imaging of symmetric and asymmetric divisions of isolated NSPCs.

Graphical abstract: A fluorescent probe for imaging symmetric and asymmetric cell division in neurosphere formation

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C4CC02974G
Article type
Communication
Submitted
22 Apr 2014
Accepted
22 May 2014
First published
22 May 2014

Chem. Commun., 2014,50, 7492-7494

Author version available

Download author version (PDF)

Permissions

Request permissions

A fluorescent probe for imaging symmetric and asymmetric cell division in neurosphere formation

S. Yun, C. Leong, X. Bi, H. Ha, Y. H. Yu, Y. L. Tan, G. Narayanan, S. Sankaran, J. Kim, S. Hariharan, S. Ahmed and Y. Chang, Chem. Commun., 2014, 50, 7492 DOI: 10.1039/C4CC02974G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements