Issue 80, 2014
From the journal:

Chemical Communications

A strain-promoted alkyne–azide cycloaddition (SPAAC) reaction of a novel EpCAM aptamer–fluorescent conjugate for imaging of cancer cells†

Nithya Subramanian,ab   Jagadeesh Babu Sreemanthula,a   Baghavathi Balaji,c   Jagat R. Kanwar,b   Jyotirmay Biswasd  and  Subramanian Krishnakumar*ad  

* Corresponding authors

a Department of Nanobiotechnology, Kamalnayan Bajaj Research Institute, Vision Research Foundation, Chennai, India
E-mail: drkrishnakumar_2000@yahoo.com

b Nanomedicine Laboratory of Immunology and Molecular Biomedical Research (N-LIMBR), School of Medicine (SoM), Molecular and Medical Research (MMR) Strategic Research Centre, Faculty of Health, Deakin University, Geelong, Victoria, Australia

c The Department of Sciences and Humanities, Vignan University, Vadlamudi, Andhra Pradesh, India

d L & T Department of Ocular Pathology, Kamalnayan Bajaj Research Institute, Vision Research Foundation, Chennai, India

Abstract

For the first time, a novel EpCAM aptamer (SYL3C)-DIBO-AF594 fluorescent conjugate was synthesised by bioorthogonal chemistry utilizing a strain promoted alkyne–azide cycloaddition (copper free click) reaction (SPAAC). The ligation efficiency of SPAAC was improved by freeze–thaw cycles. The obtained conjugate showed target specific binding and aided in the imaging of various EpCAM positive cancer cell lines like MCF7, MDAMB453, Weri-RB1 and PC3.

Graphical abstract: A strain-promoted alkyne–azide cycloaddition (SPAAC) reaction of a novel EpCAM aptamer–fluorescent conjugate for imaging of cancer cells

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C4CC02996H
Article type
Communication
Submitted
23 Apr 2014
Accepted
27 May 2014
First published
19 Jun 2014

Chem. Commun., 2014,50, 11810-11813

Author version available

Download author version (PDF)

Permissions

Request permissions

A strain-promoted alkyne–azide cycloaddition (SPAAC) reaction of a novel EpCAM aptamer–fluorescent conjugate for imaging of cancer cells

N. Subramanian, J. B. Sreemanthula, B. Balaji, J. R. Kanwar, J. Biswas and S. Krishnakumar, Chem. Commun., 2014, 50, 11810 DOI: 10.1039/C4CC02996H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements