Issue 69, 2014
From the journal:

Chemical Communications

A label-free double-amplification system for sensitive detection of single-stranded DNA and thrombin by liquid chromatography-mass spectrometry†

Wenbo Zhao,a   Zhen Qin,a   Chengsen Zhang,a   Meiping Zhao*a  and  Hai Luo*a  

* Corresponding authors

a Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
E-mail: mpzhao@pku.edu.cn, hluo@pku.edu.cn
Fax: +86-10-62751708

Abstract

A label-free double amplification system has been developed by using a ternary DNA probe containing the poly(adenine-thymine) sequence assisted by exonuclease III degradation. The method achieved more than 600-fold signal amplification and allowed sensitive detection of single-stranded DNA and thrombin at the pM level by using liquid chromatography/mass spectrometry.

Graphical abstract: A label-free double-amplification system for sensitive detection of single-stranded DNA and thrombin by liquid chromatography-mass spectrometry

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Article information

DOI
https://doi.org/10.1039/C4CC03559C
Article type
Communication
Submitted
12 May 2014
Accepted
09 Jul 2014
First published
09 Jul 2014

Chem. Commun., 2014,50, 9846-9848

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A label-free double-amplification system for sensitive detection of single-stranded DNA and thrombin by liquid chromatography-mass spectrometry

W. Zhao, Z. Qin, C. Zhang, M. Zhao and H. Luo, Chem. Commun., 2014, 50, 9846 DOI: 10.1039/C4CC03559C

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