Issue 88, 2014
From the journal:

Chemical Communications

Biological stability and activity of siRNA in ionic liquids†

Romiza R. Mazid,a   Upulie Divisekera,a   Wenjuan Yang,a   Vijayaraghavan Ranganathan,b   Douglas R. MacFarlane,b   Christina Cortez-Jugoc  and  Wenlong Cheng*a  

* Corresponding authors

a Department of Chemical Engineering, Monash University, Clayton 3800, VIC, Australia
E-mail: wenlong.cheng@monash.edu

b School of Chemistry, Monash University, Clayton 3800, VIC, Australia

c Department of Mechanical and Aerospace Engineering, Monash University, Clayton 3800, VIC, Australia

Abstract

We report substantially enhanced biological stability of siRNA in a hydrated ionic liquid (IL) based on buffered choline dihydrogen phosphate (CDP). The results show that hydrated CDP IL substantially prolongs the shelf-life of siRNA for up to three months in the presence of RNase A. Remarkably, siRNA stored in the IL remained active and eGFP siRNA exhibited clear gene knockdown effects in eGFP-expressing HeLa cells.

Graphical abstract: Biological stability and activity of siRNA in ionic liquids

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Article information

DOI
https://doi.org/10.1039/C4CC05086J
Article type
Communication
Submitted
03 Jul 2014
Accepted
05 Sep 2014
First published
05 Sep 2014

Chem. Commun., 2014,50, 13457-13460

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Biological stability and activity of siRNA in ionic liquids

R. R. Mazid, U. Divisekera, W. Yang, V. Ranganathan, D. R. MacFarlane, C. Cortez-Jugo and W. Cheng, Chem. Commun., 2014, 50, 13457 DOI: 10.1039/C4CC05086J

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