Issue 3, 2014
From the journal:

Green Chemistry

Modular multi-enzyme cascade process using highly stabilized enzyme microbeads†

Jinyang Chung,‡ab   Ee Taek Hwang,‡c   Ji Hoon Kim,a   Byoung Chan Kim*b  and  Man Bock Gu*a  

* Corresponding authors

a College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-gu, Seoul, Republic of Korea
E-mail: mbgu@korea.ac.kr
Fax: +82-2-928-3555
Tel: +82-2-3290-3417

b Center for Environment, Health and Welfare Research, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul, Republic of Korea
E-mail: bchankim@kist.re.kr
Tel: +82-2-958-5877

c Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, Denickestrasse 15, D-21073 Hamburg, Germany

Abstract

A highly stable, homogeneous and magnetically separable enzyme microbead (EMB) comprised of branched-polymer/silica-shell hybrid microbeads is demonstrated. In addition, an EMB-based modular in vitro cascade reaction system is successfully implemented to the multi-enzymatic reactions with reusability and tractability.

Graphical abstract: Modular multi-enzyme cascade process using highly stabilized enzyme microbeads

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Article information

DOI
https://doi.org/10.1039/C3GC41737A
Article type
Communication
Submitted
23 Aug 2013
Accepted
08 Jan 2014
First published
09 Jan 2014

Green Chem., 2014,16, 1163-1167

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Modular multi-enzyme cascade process using highly stabilized enzyme microbeads

J. Chung, E. T. Hwang, J. H. Kim, B. C. Kim and M. B. Gu, Green Chem., 2014, 16, 1163 DOI: 10.1039/C3GC41737A

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