Issue 31, 2014
From the journal:

RSC Advances

A thermally switchable chromatographic material for selective capture and rapid release of proteins and nucleotides†

Zongjian Liu,‡ab   Rui Su,‡ac   Xiao Liang,a   Yanli Liang,a   Yulin Deng,*a   Yujuan Lia  and  Rongji Dai*a  

* Corresponding authors

a School of Life Science, Beijing Institute of Technology, Beijing 100081, China
E-mail: deng@bit.edu.cn, dairongji@bit.edu.cn
Fax: +86 10 6846 7208
Tel: +86 10 6894 9331

b China-America Institute of Neuroscience, Luhe teaching hospital, Capital Medical University, Beijing 101100, China

c School of Chemical Engineering and Environment, North University of China, Taiyuan 030051, China

Abstract

We report a switchable chromatographic material for the selective capture-release of proteins with simpler adjustment of the temperature instead of the conventional need to change the mobile phase. This novel strategy greatly increased the number of LC/MS/MS-identified proteins from human serum, as well as their scores and coverage ratios.

Graphical abstract: A thermally switchable chromatographic material for selective capture and rapid release of proteins and nucleotides

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Article information

DOI
https://doi.org/10.1039/C3RA41454J
Article type
Communication
Submitted
25 Mar 2013
Accepted
20 Mar 2014
First published
21 Mar 2014

RSC Adv., 2014,4, 15830-15834

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A thermally switchable chromatographic material for selective capture and rapid release of proteins and nucleotides

Z. Liu, R. Su, X. Liang, Y. Liang, Y. Deng, Y. Li and R. Dai, RSC Adv., 2014, 4, 15830 DOI: 10.1039/C3RA41454J

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