Issue 49, 2014
From the journal:

RSC Advances

Nanoporous scaffold for DNA polymerase: pore-size optimisation of mesoporous silica for DNA amplification†

Shun-ichi Matsuura,‡*a   Tomoya Baba,‡b   Manami Chibaa  and  Tatsuo Tsunodaa  

* Corresponding authors

a Research Center for Compact Chemical System, National Institute of Advanced Industrial Science and Technology (AIST), 4-2-1 Nigatake, Miyagino-ku, Sendai, Miyagi 983-8551, Japan
E-mail: matsuura-shunichi@aist.go.jp
Fax: +81-22-237-5215
Tel: +81-22-237-3039

b Transdisciplinary Research Integration Center, Research Organization of Information and Systems (ROIS), National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan

Abstract

Composites of thermostable DNA polymerase and mesoporous silicas with different pore diameters were used for DNA amplification. The enzyme was selectively immobilised on the mesopores, and DNA amplification activity was retained by regulating the pore size of the mesoporous silicas.

Graphical abstract: Nanoporous scaffold for DNA polymerase: pore-size optimisation of mesoporous silica for DNA amplification

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Article information

DOI
https://doi.org/10.1039/C4RA02725F
Article type
Communication
Submitted
28 Mar 2014
Accepted
30 May 2014
First published
03 Jun 2014

RSC Adv., 2014,4, 25920-25923

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Nanoporous scaffold for DNA polymerase: pore-size optimisation of mesoporous silica for DNA amplification

S. Matsuura, T. Baba, M. Chiba and T. Tsunoda, RSC Adv., 2014, 4, 25920 DOI: 10.1039/C4RA02725F

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