In vitro osteogenesis of mesenchymal stem cells promoted by the release of La3+ and SiO44− from sheet-shaped lanthanum dropped hexagonal mesoporous silicon

Abstract

In this study, in vitro osteogenesis was successfully promoted in mouse mesenchymal stem cells (BMSCs) by the release of La³⁺ and SiO₄⁴⁻ from a sheet-shaped lanthanum dropped hexagonal mesoporous silica (La-HMS). In comparison with previous nano-sphere shaped La-HMS, the sheet-shaped morphology was similar to the inorganic component of bone, which could provide better toughness during bone repair and regeneration. The successful incorporation of La³⁺ into the HMS was confirmed by the results of XRD and ICP-AES. In vitro proliferation was verified by CCK-8 and osteogenesis was verified by an alkaline phosphatase (ALP) activity assay, ALP staining, a calcium secretion assay and collagen secretion analysis. Considering the renewable source and highly proliferative nature of BMSCs, the achievement of engineered BMSC osteogenesis with this La-HMS could allow new applications in major bone repair and regeneration.