Preparation of ionic liquid polymer materials and their recognition properties for proteins

Abstract

In this paper, two polymer materials were synthesized by using 1-allyl-3-butylimidazolium chloride ([ABIM][Cl]) or 1-vinyl-3-octylimidazolium bromide ([VOIM][Br]) as functional monomers, and arcylamide (AAm) as a co-functional monomer. Bovine serum albumin (BSA), bovine hemoglobin (BHb), lysozyme (Lyz), and cytochrome C (Cyt C) that have different properties were selected as model proteins to investigate the absorption and separation abilities of ionic liquids polymer materials. The selective absorption properties of two polymer materials for proteins were compared for the first time. The results showed that [ABIM][Cl] ionic liquid polymer material had a high binding capacity for BHb (828.5 mg g⁻¹), and [VOIM][Br] polymer material possessed a high binding capacity for BSA (804.7 mg g⁻¹). Different proteins can be separated by altering the cations of the ionic liquid. In addition, the structure, morphology and thermal properties of ILs polymer materials were characterized by scanning electron microscope (SEM), fourier transform infrared (FT-IR) spectroscopy, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). These results indicated that the IL polymer materials created a tendency for the partition of proteins efficiently and may be applied in medical diagnosis, proteomics, biotechnology and sensors broadly.