Issue 87, 2014
From the journal:

RSC Advances

Copper-catalysed cycloaddition reactions of nitrones and alkynes for bioorthogonal labelling of living cells†

Allison R. Sherratt,a   Mariya Chigrinova,a   Craig S. McKay,ab   Louis-Philippe B. Beaulieu,ab   Yanouchka Rouleaua  and  John Paul Pezacki*ab  

* Corresponding authors

a Life Sciences Division, National Research Council of Canada, Ottawa, Ontario, Canada
E-mail: John.Pezacki@nrc-cnrc.gc.ca

b Department of Chemistry, University of Ottawa, Ottawa, Canada

Abstract

An adapted biocompatible version of the Kinugasa reaction, the copper-catalysed alkyne-nitrone cycloaddition followed by rearrangement (CuANCR), was developed for live-cell labelling. CuANCR labelling was demonstrated for both mammalian and bacterial cells. A method for metabolic incorporation of the nitrone group is also described.

Graphical abstract: Copper-catalysed cycloaddition reactions of nitrones and alkynes for bioorthogonal labelling of living cells

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Article information

DOI
https://doi.org/10.1039/C4RA07851A
Article type
Communication
Submitted
30 Jul 2014
Accepted
16 Sep 2014
First published
17 Sep 2014

RSC Adv., 2014,4, 46966-46969

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Copper-catalysed cycloaddition reactions of nitrones and alkynes for bioorthogonal labelling of living cells

A. R. Sherratt, M. Chigrinova, C. S. McKay, L. B. Beaulieu, Y. Rouleau and J. P. Pezacki, RSC Adv., 2014, 4, 46966 DOI: 10.1039/C4RA07851A

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