Functional hybrids of layered double hydroxides with hemin: synergistic effect for peroxynitrite-scavenging activity

Abstract

Hemin has been successfully modified onto the surface of CuAl layered double hydroxide nanosheets by a simple coprecipitation process, which afforded a hemin modified CuAl layered double hydroxide (H-LDH) hybrid functional material that exhibited protective effects against the harmful ONOO⁻. The obtained products were characterized by X-ray powder diffraction, scanning electron microscopy, transmission electron microscopy and Fourier transform infrared spectroscopy, which showed that the samples had hexagonal symmetry structure with a mean lateral size of 1 μm, on the surface of which were adsorbed round particles with a diameter of about 300 nm. A detailed inhibition study on ONOO⁻-mediated nitration reactions indicated that the interaction between hemin and LDHs results in a synergistic effect, which leads to an efficient reduction of ONOO⁻ to nitrate. The present study suggests that the H-LDHs had an efficient ONOO⁻ scavenging ability, and may well be a potent ONOO⁻ scavenger for protection of the cellular defense activity against ONOO⁻ involved diseases.