Development of a highly selective H2S fluorescent probe and its application to evaluate CSE inhibitors

Abstract

In this paper, we developed a novel fluorescent probe C359 for highly selective detection of H₂S over other relevant biothiols. C359 is designed to contain a thiol-specific cleavable disulfide bond. H₂S-mediated the disulfide cleavage and subsequent intramolecular cyclization released the masked 7-hydroxyl coumarin, displaying a remarkable fluorescence enhancement. With the promising features in hand, C359 has been applied to detect the activity of CSE (one of H₂S-producing enzyme) and build up an assay for screening CSE inhibitors. We anticipated that the enzyme assay using C359 could provide a powerful methodology for screening more potent and selective enzyme inhibitors.