Issue 7, 2014

Single molecule analysis of light-regulated RNA:spiropyran interactions

Abstract

The photo-regulated interactions between an RNA aptamer and photochromic spiropyran were investigated at a single-molecule level via an α-hemolysin nanopore. Upon irradiation of alternating UV/visible light, the translocation process of the RNA aptamer could be optically tuned on account of its different binding affinity with two photoisomers, spiropyran and merocyanine. This provides a general analytic model for understanding the mechanism of a photo-regulated biomolecule conformational change at a single-molecule level.

Graphical abstract: Single molecule analysis of light-regulated RNA:spiropyran interactions

Supplementary files

Article information

Article type
Edge Article
Submitted
14 Jan 2014
Accepted
06 Mar 2014
First published
07 Mar 2014

Chem. Sci., 2014,5, 2642-2646

Single molecule analysis of light-regulated RNA:spiropyran interactions

X. Zhang, J. Zhang, Y. Ying, H. Tian and Y. Long, Chem. Sci., 2014, 5, 2642 DOI: 10.1039/C4SC00134F

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