Issue 1, 2015

Rapid determination of N-methylpyrrolidine in cefepime by combining direct infusion electrospray ionisation-time-of-flight mass spectrometry with field asymmetric waveform ion mobility spectrometry

Abstract

The determination of N-methyl pyrrolidine, a potential impurity in the cephalosporin antibiotic cefepime, by direct infusion ESI combined with field asymmetric waveform ion mobility spectrometry-mass spectrometry (ESI-FAIMS-MS) is demonstrated. The addition of a chip-based FAIMS separation prior to detection by time-of-flight mass spectrometry enables selective transmission of NMP in the presence of cefepime without interference from NMP formed by CID in the mass spectrometer interface. The limits of detection and quantification of NMP in cefepime were 0.011% (w/w) and 0.036% (w/w) NMP in cefepime respectively, well below the 0.3% (w/w) threshold concentration for NMP in cefepime. The % relative standard deviation was 3.9% with linearity for standard additions in the range 0.005–0.5 μg ml−1 NMP.

Graphical abstract: Rapid determination of N-methylpyrrolidine in cefepime by combining direct infusion electrospray ionisation-time-of-flight mass spectrometry with field asymmetric waveform ion mobility spectrometry

Article information

Article type
Communication
Submitted
29 Aug 2014
Accepted
03 Nov 2014
First published
04 Nov 2014

Anal. Methods, 2015,7, 34-39

Rapid determination of N-methylpyrrolidine in cefepime by combining direct infusion electrospray ionisation-time-of-flight mass spectrometry with field asymmetric waveform ion mobility spectrometry

R. W. Smith, L. B. Cox, A. Yudin, J. C. Reynolds, M. Powell and C. S. Creaser, Anal. Methods, 2015, 7, 34 DOI: 10.1039/C4AY02026J

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