Issue 15, 2015

Rapid detection of aspergillosis in immunocompromised patients using DIMS and chemometric analysis

Abstract

Rapid diagnosis of aspergillosis is one of the most important aspects for disease control and treatment. The purpose of this study was to develop a metabolic fingerprint of serum from immunocompromised patients that can enable rapid detection of aspergillosis. Serum samples from immunocompromised patients with and without aspergillosis were analyzed by direct infusion mass spectrometry (DIMS). Data from the mass spectra were subjected to chemometric analysis using principal component analysis and partial least squares-discriminant analysis. Our method was able to efficiently distinguish patients with and without aspergillosis and predict the presence or absence of aspergillosis in unknown samples. Therefore, DIMS along with chemometric analysis seems to be a promising technique for rapid diagnosis of aspergillosis.

Graphical abstract: Rapid detection of aspergillosis in immunocompromised patients using DIMS and chemometric analysis

Article information

Article type
Paper
Submitted
04 May 2015
Accepted
27 Jun 2015
First published
10 Jul 2015

Anal. Methods, 2015,7, 6346-6351

Author version available

Rapid detection of aspergillosis in immunocompromised patients using DIMS and chemometric analysis

T. M. G. de Francisco, I. F. Zaramella, J. C. Gasparetto, L. B. Cerqueira, M. S. Piantavini, R. Pontarolo and F. R. Campos, Anal. Methods, 2015, 7, 6346 DOI: 10.1039/C5AY01146A

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