Issue 12, 2015

Terminus-free siRNA prepared by photo-crosslinking activated via slicing by Ago2

Abstract

We report the development of photo-crosslinked siRNA strands modified at each terminus with p-cyanostilbene. The siRNA was nuclease resistant and retained RNAi activity. We further studied the activation mechanism of the covalently-crosslinked siRNA. Interestingly Dicer, which is known to generate siRNA with overhanging 3′ ends from the precursor siRNA, did not cleave the crosslinked siRNA at all. Our results suggest that the activation of the crosslinked siRNAs required cleavage by Argonaute2.

Graphical abstract: Terminus-free siRNA prepared by photo-crosslinking activated via slicing by Ago2

Supplementary files

Article information

Article type
Communication
Submitted
07 Jul 2015
Accepted
23 Oct 2015
First published
03 Nov 2015

Biomater. Sci., 2015,3, 1534-1538

Author version available

Terminus-free siRNA prepared by photo-crosslinking activated via slicing by Ago2

Y. Kamiya, K. Iishiba, T. Doi, K. Tsuda, H. Kashida and H. Asanuma, Biomater. Sci., 2015, 3, 1534 DOI: 10.1039/C5BM00231A

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