Issue 9, 2015
From the journal:

Chemical Communications

Compact multi-enzyme pathways in P. pastoris†

Martina Geier,‡a   Pia Fauland,‡a   Thomas Voglb  and  Anton Glieder*b  

* Corresponding authors

a Austrian Centre of Industrial Biotechnology (ACIB GmbH), Petersgasse 14, 8010 Graz, Austria

b Institute of Molecular Biotechnology, NAWI Graz & DK Molecular Enzymology, TU Graz, Petersgasse 14, 8010 Graz, Austria
E-mail: anton.glieder@tugraz.at

Abstract

We report for the first time the functional simultaneous expression of nine genes from a single 2A peptide based polycistronic expression construct. The feasibility and arising opportunities for the biosynthetic pathway balancing for chemical production were demonstrated by the co-expression of the violacein and carotenoid biosynthesis pathways.

Graphical abstract: Compact multi-enzyme pathways in P. pastoris

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Article information

DOI
https://doi.org/10.1039/C4CC08502G
Article type
Communication
Submitted
28 Oct 2014
Accepted
03 Dec 2014
First published
03 Dec 2014
This article is Open Access
Creative Commons BY license

Chem. Commun., 2015,51, 1643-1646

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Compact multi-enzyme pathways in P. pastoris

M. Geier, P. Fauland, T. Vogl and A. Glieder, Chem. Commun., 2015, 51, 1643 DOI: 10.1039/C4CC08502G

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