A multiplex fluorophore molecular beacon: detection of the target sequence using large Stokes shift and multiple emission signal properties

Abstract

We have developed a multiplex fluorophore molecular beacon (mfMB) with fluorophores located at its end to produce unique FRET (Fluorescence Resonance Energy Transfer). It exhibited diverse fluorescence properties depending on the mixing pattern, such as large Stokes shift emission and multiple colors, namely, blue, green and red using one excitation wavelength. Our mfMB also worked in probing a target perfect matched sequence with exonuclease III.