Issue 26, 2015
From the journal:

Chemical Communications

Fluorescence-based detection of single-nucleotide changes in RNA using graphene oxide and DNAzyme†

Chaesun Hong,a   Dong-Min Kim,a   Ahruem Baek,a   Hyewon Chung,b   Woong Jungc  and  Dong-Eun Kim*a  

* Corresponding authors

a Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea
E-mail: kimde@konkuk.ac.kr
Fax: +82-2-3436-6062
Tel: +82-2-2049-6062

b Department of Ophthalmology, Konkuk University Medical Center, Seoul 134-729, Republic of Korea

c Department of Emergency Medicine, Kyung Hee University Hospital at Gangdong, Seoul 134-727, Republic of Korea

Abstract

We report a simple fluorometric method for detection of single-nucleotide changes in RNA using graphene oxide (GO) and RNA-cleaving DNAzyme. The fluorescent DNA probe (F-DNA) was annealed to RNA fragments generated by RNA cleavage with DNAzyme specific to mutant RNA. The F-DNA–RNA duplex attenuated the quenching of F-DNA fluorescence by GO.

Graphical abstract: Fluorescence-based detection of single-nucleotide changes in RNA using graphene oxide and DNAzyme

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Article information

DOI
https://doi.org/10.1039/C4CC09603G
Article type
Communication
Submitted
02 Dec 2014
Accepted
17 Feb 2015
First published
17 Feb 2015

Chem. Commun., 2015,51, 5641-5644

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Fluorescence-based detection of single-nucleotide changes in RNA using graphene oxide and DNAzyme

C. Hong, D. Kim, A. Baek, H. Chung, W. Jung and D. Kim, Chem. Commun., 2015, 51, 5641 DOI: 10.1039/C4CC09603G

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