Issue 61, 2015
From the journal:

Chemical Communications

Real-time monitoring of small biological molecules by ligation-mediated polymerase chain reaction†

Yan Zhang,‡a   Jiao Liu‡ab  and  Chun-yang Zhang*ac  

* Corresponding authors

a Single-Molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
E-mail: zhangcy@siat.ac.cn
Fax: +86-755-86392299
Tel: +86-755-86392211

b Nano Science and Technology Institute, University of Science and Technology of China, Suzhou, China

c College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, China
E-mail: cyzhang@sdnu.edu.dn
Fax: +86 0531-86180024
Tel: +86 0531-86182545

Abstract

We develop a ligation-mediated polymerase chain reaction (PCR) for real-time detection of small biological molecules in a high-throughput format. This method is extremely sensitive with a detection limit of as low as 18.8 fM for ATP and 17.3 fM for NAD+, and it can discriminate target molecules from their analogues as well.

Graphical abstract: Real-time monitoring of small biological molecules by ligation-mediated polymerase chain reaction

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Article information

DOI
https://doi.org/10.1039/C5CC04269K
Article type
Communication
Submitted
24 May 2015
Accepted
22 Jun 2015
First published
22 Jun 2015

Chem. Commun., 2015,51, 12270-12273

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Real-time monitoring of small biological molecules by ligation-mediated polymerase chain reaction

Y. Zhang, J. Liu and C. Zhang, Chem. Commun., 2015, 51, 12270 DOI: 10.1039/C5CC04269K

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