Issue 10, 2015
From the journal:

Physical Chemistry Chemical Physics

Direct observation of the growth and shrinkage of microtubules by single molecule Förster resonance energy transfer†

Prasenjit Mondal,‡a   Shyamtanu Chattoraj,‡b   Rajdeep Chowdhury,‡b   Debmalya Bhunia,a   Surajit Ghosh§*a  and  Kankan Bhattacharyya*b  

* Corresponding authors

a Chemistry Division, CSIR-Indian Institute of Chemical Biology, Jadavpur, Kolkata-700032, India
E-mail: sghosh@iicb.res.in

b Department of Physical Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Kolkata-700032, India
E-mail: pckb@iacs.res.in

Abstract

Single molecule Förster resonance energy transfer (FRET) has been applied, for the first time, to monitor the growth (polymerization) and the shrinkage (depolymerization) of the dynamic microtubules, employing EGFP (attached to Mal3) as a donor and alexa-568 bound to tubulin as an acceptor.

Graphical abstract: Direct observation of the growth and shrinkage of microtubules by single molecule Förster resonance energy transfer

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Article information

DOI
https://doi.org/10.1039/C4CP06031H
Article type
Communication
Submitted
23 Dec 2014
Accepted
27 Jan 2015
First published
28 Jan 2015

Phys. Chem. Chem. Phys., 2015,17, 6687-6690

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Direct observation of the growth and shrinkage of microtubules by single molecule Förster resonance energy transfer

P. Mondal, S. Chattoraj, R. Chowdhury, D. Bhunia, S. Ghosh and K. Bhattacharyya, Phys. Chem. Chem. Phys., 2015, 17, 6687 DOI: 10.1039/C4CP06031H

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